the effect of ascorbic acid and garlic administration on lead-induced apoptosis in rat offspring's eye retina

Authors

الناز خرداد

elnaz khordad علیرضا فاضل

alireza fazel علیرضا ابراهیم زاده بیدسکان

alireza ebrahimzadeh bideskan

abstract

introduction: lead toxicity induces retinal cell apoptosis. vitamin c and garlic may decrease lead-induced apoptosis. this study was undertaken to investigate vitamin c and garlic protective effects on lead-induced apoptosis in eye retina. methods: pregnant wistar rats (n = 72) were divided randomly into 9 groups: (l) treated rats with lead acetate in drinking water and (l+aa) with leaded water and vitamin c intraperitoneally(l+g), the rats received leaded-water and garlic juice via gavage (l+aa+g) treated rats with leaded water, ascorbic acid, and garlic juice, (aa) with ascorbic acid, and (g) with garlic juice (aa+g) treated rats with vitamin c and garlic juice and (sh) with tap water plus normal hydrogen chloride (hcl) and glucose normal (n). after 21-day lactation, blood lead level (bll) in rats was measured, and then their offspring and the rat offspring&apos;s eyes were removed and processed for using tunel method. tunel positive cells in the eye retina were counted and all groups were compared. results: bll increased in l group compared to the control groups and decreased significantly in l + g, l + aa, and l+ aa + g groups compared to l group (p<0.05). tunell positive cell number in eye retina significantly increased in l group compared to control groups (p<0.05) and decreased in l+ g, l+ aa, and l+aa + g groups compared to l group (p<0.05). conclusion: garlic juice and ascorbic acid administration during pregnancy and lactation may protect lead-induced apoptosis in rat offspring&apos;s eye retina.

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Journal title:
iranian biomedical journal

جلد ۱۷، شماره ۴، صفحات ۲۰۶-۲۱۳

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